Evaluation of the in vitro protein bioaccessibility of several microalgae and cyanobacteria as potential dietary ingredients in gilthead seabream (Sparus aurata) juveniles

Abstract This work addresses the evaluation in vitro of different marine ( Dunaliella salina REC-0214B and Microchloropsis gaditana REC-0251B) freshwater Anabaena sp. BEA-0300B, Arthrospira platensis BEA-0007B, Chlorella vulgaris BEA-0753B, Spirogyra BEA-0666B) microalgae cyanobacteria as potential dietary ingredients aquafeeds. For this purpose, total protein content, amino acid composition, presence protease inhibitors were evaluated. In addition, bioaccessibility was estimated using a species-specific assay Sparus aurata digestive enzymes. Overall, all showed high content ranging from 25 to 61%, balanced essential non-essential (from 0.81 0.95). The inhibition confirmed absence whatever considered. Finally, assays differences degree hydrolysis with values for coefficient degradation 49.4% 85.5% D. . Similarly, amount free acids released microalgal biomass 12.8 20.8 g L-leucine equivalents (100 protein) −1 ), well their qualitative profile varied among species, although can be considered cases. conclusion, results obtained revealed that, even if significant observed species terms susceptibility hydrolysed by S. enzymes, evaluated presented an adequate profile.